Rana Ezzeddini
Department of Clinical biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Title: High Expression of HIF1α is a predictor in patients with gastric adenocarcinoma
Biography
Biography: Rana Ezzeddini
Abstract
Background: Gastric adenocarcinoma has been known as one of the virulent diseases with weak prognosis. New findings of gastric cancers according to histologic features and molecular phenotypes improve early diagnosis, prevention and treatment. Hypoxia inducible factor 1α (HIF1α) are expressed in some human cancers and were previously suspected of promoting tumor growing and poor patient outcome. The objective of this study is to investigate the expression of HIF1α protein in adenocarcinoma tissues.
Materials & Methods: We selected 105 gastric cancer patients and 130 healthy control with the age range of 30-80 in order to carry out this study. The ethical committee of Tarbiat Modares University (Tehran, Iran) authorized this study. The study was explained to the participants and Informed consent was obtained from all individuals. Formalin-fixed, paraffin wax-embedded sections were cut at approximately 5 μm. Immunohistochemical staining for HIF1α were performed in all samples. Image J software was used for microscopic investigation and comparing the outcomes. We used t test for data analysis. P values less than 0.05 was considered as statistically significant difference in all cases.
Results: Our findings indicated significant upregulation of HIF1α expression in gastric adenocarcinoma tissues (0.25±0.07) compare to control group (0.18±0.04) ( P = 0.0001).
Conclusion: As a result, the present study suggested that increased HIF1α were involved in progression of gastric adenocarcinoma cells that can be used for distinguished classification.
Keywords: Gastric adenocarcinoma, Immunohistochemistry, HIF1α