17^th European Pathology Congress May 09-10, 2019 Amsterdam, Netherlands

Biography:

Maria Teresa Mascellino has completed her MD in Rome during the period of 1980 and specialization studies in Microbiology and Infectious Diseases from Sapienza University of Rome, Italy. She works as Aggregate Professor in the Department of Public Health and Infectious Diseases. She has published more than 100 papers in reputed journals and has been serving as an Editorial Board Member of repute. She is an Editor of three books and Reviewer for many important scientific international Journals and Research Projects from Ministry of University and Scientific Research. She has attended national and international conferences as a Speaker presenting relevant research topics

Abstract:

Aim of study is to evaluate the antibiotic resistance and the eradication rate in 40 patients with Helicobacter pylori (Hp) infection presenting gastric symptoms that required assessment with diagnostic endoscopy, who failed first line therapy. The patients all positive to urease test, were treated with the quadruple therapy of PPI, bismuth, metronidazole (MZ) and tetracycline (TE) for 14 days consistently to the high clarithromycin (CLA)- resistance level. Eradication status was determined by the 13C-urea breath test. The resistance to CLA and MZ resulted to be very high (50% and 65%, respectively) due to their large use. Amoxicillin, TE and levofloxacin showed 3%, 5% and 25% of resistance respectively. Genetic resistance has been studied for CLA and TE. Mixed Hp infections were demonstrated by the presence of different antimicrobial susceptibility patterns contemporarily. The overall eradication rate resulted as being 82% strictly depending for each patient on the number of gastric districts colonized by Hp. The CLA-resistance levels and mainly the local susceptibility resulted to be crucial in order to establish a correct therapy. The genotypic-resistance is useful in case of absence of live bacteria, contamination and for identifying mixed infections that represent a real problem possibly leading to a resistance underestimation. The real-time PCR detected the resistant population at a very low concentration not detectable by phenotypic tests which primarily show susceptible bacteria. The use of genotypic tests directly on the clinical specimens could predict the antibiotic resistance addressing changes in previous failure treatments. The bismuth quadruple therapy resulted to be effective in the Hp eradication rate.

Biography:

Doughty ES has completed her Bachelor of Science (2007) in Biomedical Engineering from Tulane University in New Orleans, Louisiana, USA. She has obtained both Master of Science (2011) and a Doctorate (2014) from the University of California, Davis, in Biomedical Engineering with an emphasis in Biomechanics. She has switched to a career in Medicine and obtained a Master of Pathology (2018) concurrent with her Medical Education at the University of Vermont. She will obtain her Doctorate in Medicine (2019) this spring and will continue her Resident Physician education in Anatomic and Clinical Pathology in July

Abstract:

During female embryologic development, the absence of testosterone promotes mesonephric (Wolffian) duct involution. Mesonephric remnants persist and can, rarely, undergo transformation to mesonephric adenocarcinoma (MA), an aggressive neoplasm difficult to differentiate from female adnexal tumor of probable Wolffian origin (FATWO), a neoplasm of uncertain origin. MA and FATWO have similar morphologic and immunohistochemical (IHC) features, suggesting that these may represent the same entity. We present a case of FATWO and a case of MA, examining differences in morphology/IHC findings. Case 1: Seventy four-year-old woman with a large pelvic mass involving the left adnexa and colonic serosa. Microscopically, the infiltrative mass displayed retiform architecture with intraluminal eosinophilic acellular material, significant mitotic activity, and necrosis. IHC results: WT-1 positive, PAX-8 negative and GATA3 negative. The tumor was diagnosed as malignant FATWO. Case 2: Sixty one-year-old woman with bilateral ovarian masses, omental caking and ascites. The tumor was heterogeneous with prominent retiform and papillary architectures, clear cell change, abundant mitoses and no necrosis. IHC results: WT-1 negative, PAX-8 positive and GATA3 focal positivity. The tumor was initially diagnosed as malignant FATWO. The patient initially responded to platinum-based adjuvant chemotherapy, but recurred four months following treatment. Subsequent genetic tumor testing on the primary surgical debulking specimen demonstrated a NRAS mutation, consistent with MA. We propose that MA and FATWO are distinct entities and that the WT-1/PAX-8/GATA3 immunoreactivity pattern and NRAS mutation can help in the diagnosis of FATWO and MA. These two cases highlight the utility of IHC and genetic analysis in the distinction of these two entities.

Biography:

Nadia Sukhram has completed her BSc in Medical Technology from St. John’s University, USA, and holds a MSc in Public Health from Touro University, USA. She is the Operations Manager for Anatomic Pathology at Northwell Health Long Island Jewish Hospital and the Operations Manger for Flow Cytometry at Northwell Health North Shore University Hospital..

Abstract:

A major challenge to understanding the hematopathology service line stems from inadequate delving into the backbone of the field itself – the flow cytometry lab. The talk will take the audience briefly back into history when flow cytometry debued into clinical patient care and will transition to what it is today. It will cover some of the most frequesntly ordered tests done by flow cytometry along with the instrumentation and challenges to provide fast and accurate results.

Biography:

Abstract:

Bartter’s syndrome (BS) is a rare autosomal recessive syndrome that is characterized by hypochalemic metabolic alkalosis, high renin and aldosterone plasma levels, and high levels of prostaglandins in blood and urine. In this case, we present a fi ve months old female child admitted to our hospital with the complaints of vomiting, diarrhea and failure to thrive. On admission to our hospital the patient was mildly dehydrated and weight gain was detected 250 g/month for the last two months. Blood counts, urea, creatinine, LFT, serum calcium, magnesium, phosphorous and thyroid function tests levels were normal, but blood gas revealed metabolic alkalosis with hyponatraemia, hypokalemia and hypochloremia. Cystic fi brosis (CF) was eliminated by negative sweat test. After the fl uid and electrolyte correction the patient was discharged. During the control polyclinic examination no weight gain was detected and laboratory fi ndings revealed metabolic alkalosis with hyponatraemia, hypokalemia and hypochloremia with increased renin levels. Based on the clinical picture and laboratory data she was diagnosed as BS and started on oral KCL supplementations.Gradually child showed improvement at weight gain.

Biography:

Abstract:

Biography:

Michelle Maharaj currently employed as a Senior Medical Technologist at NHLS in Durban South Africa. Her Qualifications includes a Diploma in Clinical Pathology, a B-Tech Degree in Biomedical Technology and SANAS 17025 for QA/QC. She was HPSCA registered in Clinical Pathology, with extensive skills in all categories including differential counts. Her other field of interest include Quality Assurance Management systems and accreditation of Laboratories

Abstract:

Background & Objectives: Globally, tuberculosis is a disease of major public health concern. The Human Immunodeficiency Virus (HIV) epidemic has increased the incidence of infection with nontuberculous mycobacteria (NTM). Differentiation of Mycobacterium tuberculosis complex (MTBC) isolates from NTM is critical for accurate management and improved patient outcomes. MPT64 is a mycobacterial protein secreted only by MTBC and has been shown to differentiate MTBC from NTM. BD MGIT TBc identification test (TBc ID) is a cost effective, rapid immunochromatographic assay for qualitative detection of MPT64 antigen. The BD TBc ID kit is currently validated for use on MGIT broth media. We evaluated the performance of the TBc ID for rapid identification of MTBC in samples cultured on Middlebrook 7H11 solid media.

Method: Twenty three clinical isolates (14 sputum; five blood cultures; two gastric washings; one bronchial lavage and one CSF) and two ATCC control strains (25177 and 13950) were cultured on solid media. Colonies from 7H11 plates were emulsified in 200 μl of 7H9 broth and 100 μl was then added to the sample window to observe the development of a purple band within 15 minutes. These results were compared with Hain Genotype MTBDRplus V2 assay. Interpretation of both tests was done in accordance to the manufacture guidelines.

Results: Of the 23 samples, 18 were MPT64ag positive and five were negative. For all 18 positive samples, the MTBDRplus V2 assay confirmed the presence MTBC. The five negative samples were also negative on the MTBDRplus V2 assay. This comparison demonstrated 100% concordance between the MTBDRplus V2 assay and BD MGIT™ TBc identification test for identification of MTBC.

Conclusion: Whilst the numbers were small, our results showed a 100% correlation between the two tests demonstrating preliminary suitability of BD MGIT TBc identification test for rapid identification of MTBC from MB 7H11 colonies.