John Zhong
University of Southern California, CA 90089, USA
Title: Single-cell Molecular Analysis Reveal A Novel Molecular Pathway in Glioblastoma
Biography
Biography: John Zhong
Abstract
Molecular analysis has tranformed pathology from the morphological age into the molecular era. This transission is similar to the transisson from analog to digital TV. With molecular profiling, pathology is more precise and quantitative. However, tumor heterogeneity remains a major hurdle for obtaining the molecular profile of cancer. In this study, we apply single-cell technology to overcome this hurdle and obtain a molecular profile of a tumor stem cell from a glioblastoma multiforme (GBM) patient. We obtain the initial diagnostic biopsy from a male patient and a relapse biopsy from the same patient. We first enriched tumor stem cells by organ slide culture with the diagnosis biopsy, then perform single-cell RNA-seq on the cultured cells (enriched for tumor stem cells). Because the relapse tumor is generated from the tumor stem cells which is rare in the initial (diagnosis) tumor, but becomes majority in relapse. Therefore, single-cells carrying mutations detected in the relapse biopsy but not in the diagnosis biopsy (too rare to be detected) are the tumor initiation cells (tumor stem cells). With this approch we reveal a novel molecule patyway in the GBM involveing multiple memeber of the P53 pathway.